Beta-Galactosidase Assay: Gene Expression Analysis
The beta-galactosidase assay is a molecular biology technique used to study gene expression and promoter activity. It employs the LacZ reporter gene from the lac operon of Escherichia coli, which encodes the enzyme beta-galactosidase. When the LacZ gene is expressed, beta-galactosidase is produced and can be detected using colorimetric, fluorometric, or luminescent assays. By measuring…